| 货号 | AF8519 |
| 别名 | EC 2.7.1.40; PK1; PK1PKRL; PKL; PKR; Pyruvate Kinase 1; pyruvate kinase isozyme R/L; pyruvate kinase isozymes R/L; pyruvate kinase type L; pyruvate kinase, liver and blood cell; pyruvate kinase, liver and RBC; Red cell/liver pyruvate kinase; RPK; R-type/L-type pyruvate kinase | 全称 | Pyruvate Kinase, Liver And RBC |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1:1000 dilution) |
| 目标/特异性 | Detects human, mouse, and rat PKLR in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human PKM-2 is observed. |
| 使用方法 | Western Blot: 1:1000 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5313 (Human); 18770 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PKLR Leu47-Ser574 Accession # P30613 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 背景 | Human PKLR (Pyruvate kinase PKLR) is a 60 kDa, cytosolic red cell/liver pyruvate kinase that belongs to the pyruvate kinase family of proteins. It is 574 amino acids (aa) in length, and contains two Allosteric activator binding regions. PKLR is allosterically activated by fructose 1,6-bisphosphate. Pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia. Additional shorter isoform apparently exists. It is 543 aa in length and shows a two aa substitution for aas 1-33. Human PKLR is 93% aa identical to mouse and rat PKLR. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat PKLR by Western Blot. Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat PKLR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8519) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKLR at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
