| 货号 | AF8178 |
| 别名 | Hexokinase 1/2 |
| 反应种属 | Human |
| 应用 | Western Blot(1:1000 dilution) Immunohistochemistry(1:200 dilution) |
| 目标/特异性 | Detects human Hexokinase 1 and human Hexokinase 2 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1:1000 dilution Immunohistochemistry: 1:200 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Hexokinase 1 and E. coli-derived recombinant human Hexokinase 2 Phe11-Ser917 and Phe11-Arg917 Accession # P19367 & P52789 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | 1. Aleshin, A.E. et al. (1998) Structure 6:39-50 2. Takeda, J. et al. (1993) J. Biol. Chem. 268:15200. 3. Lange, A.J. et al. (1991) Biochem. J. 277:159–163. 4. Magnani, M. et al., J. Biol. Chem. 266: 502. 5. Nishi, S. et al. (1988) Biochem. Biophys. Res. Commun. 157:937. 6. Magnani, M. et al. (1992) Biochem. J. 285:193. 7. Van Wijk R. et al. (2003) Blood 101:345. 8. Wu, Z.L. (2011) PLoS One 6:e23172. |
Detection of Human Hexokinase 1/2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Hexokinase 1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8178) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Hexokinase 1/2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Hexokinase 1/2 in Human Brain Medulla Tissue. Hexokinase 1/2 was detected in immersion fixed paraffin-embedded sections of human brain medulla tissue using Rabbit Anti-Human Hexokinase 1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8178) at 1:200 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
