| 货号 | AF8015 |
| 别名 | DKFZp434L134; KOX25; NY-REN-21; Renal carcinoma antigen NY-REN-21; ZFP38; zfp-38; ZFP383; zinc finger and SCAN domain containing 21; zinc finger and SCAN domain-containing protein 21; zinc finger protein 38 (KOX 25); Zinc finger protein 38 homolog; zinc finger protein 38; zinc finger protein NY-REN-21 antigen; Zipro1; ZNF38; ZNF38DKFZp686H10254 | 全称 | Zinc Finger and SCAN Domain Containing 21 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human ZSCAN21 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7589 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ZSCAN21 Asn139-Asp223 Accession # Q9Y5A6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ZSCAN21 (Zinc finger and SCAN domain-containing protein 21; also Zfp-38, NY-REN-21, Zipro1 and Ru49) is a nuclear member of the multi-finger SCAN box protein family. It is expressed in select cell types, including cerebellar granule cells, Schwann cells, cerebral cortical and sympathetic neurons, round spermatids, and cells of the hair follicles plus sebaceous glands. It may have multiple functions, one of which is to initiate transcription of neuronal alpha -synuclein, while a second may be to promote granule cell proliferation. Human ZSCAN21 is 473 amino acids (aa) in length. It contains one SCAN (SREZBP, Ctfin51, AW-1/ ZNF174, and Number 18) domain (aa 45-127) that mediates dimerization, followed by seven consecutive C2H2-type zinc-finger regions (aa 277-466). ZSCAN21 is known to form both noncovalent homodimers and heterodimers with SCAND1. There is one potential isoform variant that shows a 188 aa substitution for aa 263-473. Over aa 139-223, human ZSCAN21 shares 54% aa sequence identity with mouse ZSCAN21. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human ZSCAN21 by Western Blot. Western blot shows lysates of Caki‑2 human clear cell carcinoma epithelial cell line, COLO 205 human colorectal adenocarcinoma cell line, and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ZSCAN21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8015) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZSCAN21 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
