| 货号 | AF4844 |
| 别名 | 66 kDa neurofilament protein; alpha-Inx; FLJ18662; FLJ57501; INA; internexin neuronal intermediate filament protein, alpha; MGC12702; NEF5; neurofilament 5 (66kD); Neurofilament 5; Neurofilament-66; neurofilament-66, tax-binding protein; NF-66; NF-66alpha-internexin; TXBP-1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat alpha -Internexin in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9118 (Human); 226180 (Mouse); 24503 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human alpha -Internexin Val230-Glu450 Accession # Q16352 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Alpha-Internexin ( alpha -INX; also 66 kDa neurofilament protein (NF-66)) is a 66 kDa member of the intermediate filament (IF) protein family. It was the first of two molecules named for its presumed interaction with cytoskeletal proteins. alpha -INX is one of four Class IV neuronal IF proteins. It both self-assembles, and complexes with NF-L, H and M in select cortical and cerebellar neurons. Human alpha -INX is 499 amino acids (aa) in length. It has a predicted MW of 55 kDa, but runs anomalously at 66 kDa in SDS-PAGE. alpha -INX contains one DNA-binding region (aa 10‑92), a poly-Glu segment (aa 449‑454) and three serine phosphorylation sites (Ser72/335/496). There is one 494 aa alternative splice form that shows multiple short sequence aa substitutions in the first 200 amino acids. Over aa 230‑450, human alpha -INX shares more that 96% aa identity with mouse, canine and rat alpha -INX. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat alpha ‑Internexin by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, human, mouse, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat alpha ‑Internexin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4844) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha-Internexin at approximately 66 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. |
