| 货号 | AF2628 |
| 别名 | MSI1; Musashi (Drosophila) homolog 1; musashi homolog 1 (Drosophila); musashi-1; RNA-binding protein Musashi homolog 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Musashi-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Musashi‑2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4440 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Side populations of gastrointestinal cancers are not enriched in stem cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Musashi-1 Met1-His362 Accession # O43347 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Musashi-1 is an RNA-binding protein that is expressed in the developing central nervous system. Expression is highest in proliferating multipotent neural precursor cells and decreases during neuronal differentiation (1, 2). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Musashi‑1 in Rat Cortical Stem Cells. Musashi‑1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. | |
Musashi‑1 in Mouse Cortical Stem Cells. Musashi‑1 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. | |
Musashi‑1 in Human Intestine. Musashi‑1 was detected in immersion fixed paraffin-embedded sections of human intestine using 1 µg/mL Goat Anti-Human Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
