| 货号 | AF4200 |
| 别名 | FLJ32389; Heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein, alpha-crystallin-related, B6; Hsp20; HSPB6 | 全称 | Heat Shock Protein 20 |
| 反应种属 | Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous rat HSP20/HSPB6 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 126393 (Human); 243912 (Mouse); 192245 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant rat HSP20/HSPB6 Met1-Lys162 Accession # P97541 |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Heat shock protein 20 kDa (HSP20), also known as heat shock protein, alpha -crystallin-related, B6 (HSPB6), is a 162 amino acid, 20 kDa member of the small heat shock protein family, which also includes HSP27, HSP25, and the alpha -crystallins. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. Many HSPs are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Although HSP20 expression does not increase in response to heat stress, HSP20 does translocate from the cytosol to the nucleus. The highest levels of HSP20 are found in skeletal, smooth and cardiac muscle. HSP20 may also be secreted into plasma. Rat HSP20 shares 89 % and 96 % amino acid identity with human and mouse HSP20, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Detection of Rat HSP20/HSPB6 by Western Blot. Western blot shows lysates of rat heart and skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Rat HSP20/HSPB6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4200) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSP20/HSPB6 at approximately 20 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. |
