Mouse Podocalyxin Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Mouse Podocalyxin Affinity Purified Polyclonal Ab (100 UG)

货号： AF1556 基本售价： 5076.2 元规格： -

产品信息

概述
货号	AF1556
别名	GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin-like protein 1; podocalyxin-like; PODXL
反应种属	Mouse
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5420 (Human); 27205 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance Nat Commun, 2016;7(0):12329. Species: Mouse Sample Type: Whole Tissue Application: IHC - Fresh Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice Sci Rep, 2016;6(0):30739. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Authors: Ramasamy S, Kusumbe A, Wang L, Adams R Nature, 2014;507(7492):376-80. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling. Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R Development, 2014;141(2):448-59. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria. Authors: Kumagai T, Baldwin C, Aoudjit L, Nezvitsky L, Robins R, Jiang R, Takano T Am J Pathol, 2014;184(8):2211-24. Species: Mouse Sample Type: Whole Tissue Application: ICC Fluorescence Clustered PI(4,5)P(2) accumulation and ezrin phosphorylation in response to CLIC5A. Authors: Al-Momany A, Li L, Alexander R, Ballermann B J Cell Sci, 2014;127(24):5164-78. Species: Mouse Sample Type: Whole Cells Application: ICC Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) lambda/iota. Authors: Worthmann K, Leitges M, Teng B, Sestu M, Tossidou I, Samson T, Haller H, Huber T, Schiffer M Am J Pathol, 2013;183(6):1945-59. Species: Mouse Sample Type: tissue Application: IHC Frozen Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function. Authors: Pierchala BA, Munoz MR, Tsui CC Kidney Int., 2010;78(9):868-82. Species: Mouse Sample Type: Whole Cells Application: ICC Novel regulators of kidney development from the tips of the ureteric bud. Authors: Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J J. Am. Soc. Nephrol., 2005;16(7):1993-2002. Species: Rat Sample Type: Whole Tissue Application: IHC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin Ser21-Arg402 Accession # Q9R0M4
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Li, J. et al. (2001) DNA Seq. 12(5):407. Hara, T. et al. (1999) Immunity 11(5):567.

参考图片

Detection of Mouse Podocalyxin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Podocalyxin in Mouse Thymus. Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.