| 货号 | AF911 |
| 别名 | 92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP-9; type V collagenase | 全称 | Matrix Metalloproteinase 9 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse MMP-9 in Western blots, dot blots, and human MMP-9 by immunocytochemistry. In Western blots, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4318 (Human); 17395 (Mouse); 81687 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. RAGE mediates S100A7-induced breast cancer growth and metastasis by modulating the tumor microenvironment. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human MMP-9 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Pro-MMP-9 is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9 which can degrade collagens and elastin as well as several non-ECM molecules. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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MMP‑9 in NS0 Mouse Cell Line. MMP‑9 was detected in immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 using 5 µg/mL Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
MMP‑9 in Human Ovarian Cancer Tissue. MMP‑9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
MMP‑9 in MCF‑7 Human Cell Line. MMP‑9 was detected in immersion fixed MCF‑7 human breast cancer cell line using Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
MMP‑9 in Human Ovarian Cancer Tissue. MMP‑9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
