Human SOX2 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human SOX2 Affinity Purified Polyclonal Ab (100 UG)

货号： AF2018 基本售价： 5076.2 元规格： -

产品信息

概述
货号	AF2018
别名	ANOP3; MCOPS3; MGC2413; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2
全称	Transcription Factor SOX2
反应种属	Human
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human SOX2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX1 and rhSOX17 is observed.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6657 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation Authors: Qi Xie J Clin Invest, 2016;126(7):2757-2772. Species: Human Sample Type: Cell Lysates Application: WB An essential role of SVZ progenitors in cortical folding in gyrencephalic mammals Sci Rep, 2016;6(0):29578. Species: Ferret Sample Type: Whole Tissue Application: IHC OCT-embedded Angiogenesis in the developing spinal cord: blood vessel exclusion from neural progenitor region is mediated by VEGF and its antagonists. Authors: Takahashi, Teruaki, Takase, Yuta, Yoshino, Takashi, Saito, Daisuke, Tadokoro, Ryosuke, Takahashi, Yoshiko PLoS ONE, 2015;10(1):e0116119. Species: Chicken Sample Type: tissue Application: IHC Frozen Increased cycling cell numbers and stem cell associated proteins as potential biomarkers for high grade human papillomavirus+ve pre-neoplastic cervical disease. Authors: Canham, Maurice, Charsou, Chara, Stewart, June, Moncur, Sharon, Hoodless, Laura, Bhatia, Ramya, Cong, Duanduan, Cubie, Heather, Busby-Earle, Camille, Williams, Alistair, McLoughlin, Victoria, Campbell, John D M, Cuschieri, Kate, Howie, Sarah PLoS ONE, 2015;9(12):e115379. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded DNMT-dependent suppression of microRNA regulates the induction of GBM tumor-propagating phenotype by Oct4 and Sox2. Authors: Lopez-Bertoni H, Lal B, Li A, Caplan M, Guerrero-Cazares H, Eberhart C, Quinones-Hinojosa A, Glas M, Scheffler B, Laterra J, Li Y Oncogene, 2015;34(30):3994-4004. Species: Human Sample Type: Cell Lysates Application: ChIP Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans. Authors: Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear C, Post M Stem Cell Reports, 2015;4(3):419-30. Species: Mouse Sample Type: Whole Cells Application: IHC Not Specified Mitochondrial control by DRP1 in brain tumor initiating cells. Authors: Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, Dombrowski S, Huang Z, Fang X, Shi Y, Ferguson A, Kashatus D, Bao S, Rich J Nat Neurosci, 2015;18(4):501-10. Species: Human Sample Type: Whole Tissue Application: IF MOF-associated complexes ensure stem cell identity and Xist repression. Authors: Chelmicki T, Dundar F, Turley M, Khanam T, Aktas T, Ramirez F, Gendrel A, Wright P, Videm P, Backofen R, Heard E, Manke T, Akhtar A Elife, 2014;3(0):e02024. Species: Mouse Sample Type: Cell Lysates Application: ChIP 3D imaging of Sox2 enhancer clusters in embryonic stem cells. Authors: Liu, Zhe, Legant, Wesley R, Chen, Bi-Chang, Li, Li, Grimm, Jonathan, Lavis, Luke D, Betzig, Eric, Tjian, Robert Elife, 2014;3(0):e04236. Species: Mouse Sample Type: Cell Lysate Application: ChIP Role of SOX2 in the etiology of embryonal carcinoma, based on analysis of the NCCIT and NT2 cell lines. Authors: Eini, Ronak, Stoop, Hans, Gillis, Ad J M, Biermann, Katharin, Dorssers, Lambert, Looijenga, Leendert PLoS ONE, 2014;9(1):e83585. Species: Human Sample Type: Whole Cells Application: IHC Paraffin-embedded Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells. Authors: Bayin, N Sumru, Modrek, Aram S, Dietrich, August, Lebowitz, Jonathan, Abel, Tobias, Song, Hae-Ri, Schober, Markus, Zagzag, David, Buchholz, Christia, Chao, Moses V, Placantonakis, Dimitris PLoS ONE, 2014;9(12):e116114. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. Authors: Gouti, Mina, Tsakiridis, Anestis, Wymeersch, Filip J, Huang, Yali, Kleinjung, Jens, Wilson, Valerie, Briscoe, James PLoS Biol, 2014;12(8):e1001937. Species: Human Sample Type: whole cells Application: IF Sex determining region Y-box 2 (SOX2) amplification is an independent indicator of disease recurrence in sinonasal cancer. Authors: Schrock A, Goke F, Wagner P, Bode M, Franzen A, Braun M, Huss S, Agaimy A, Ihrler S, Menon R, Kirsten R, Kristiansen G, Bootz F, Lengerke C, Perner S PLoS ONE, 2013;8(3):e59201. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Transcription factors FOXG1 and Groucho/TLE promote glioblastoma growth. Authors: Verginelli F, Perin A, Dali R, Fung K, Lo R, Longatti P, Guiot M, Del Maestro R, Rossi S, di Porzio U, Stechishin O, Weiss S, Stifani S Nat Commun, 2013;4(0):2956. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Efficient generation of human iPSCs by a synthetic self-replicative RNA. Authors: Yoshioka N, Gros E, Li H, Kumar S, Deacon D, Maron C, Muotri A, Chi N, Fu X, Yu B, Dowdy S Cell Stem Cell, 2013;13(2):246-54. Species: Human Sample Type: Whole Cells Application: IF The germinal matrices in the developing dentate gyrus are composed of neuronal progenitors at distinct differentiation stages. Authors: Sugiyama T, Osumi N, Katsuyama Y Dev Dyn, 2013;242(12):1442-53. Species: Mouse Sample Type: Whole Tissue Application: immunohistochemestry Sox2 marks epithelial competence to generate teeth in mammals and reptiles. Authors: Juuri, Emma, Jussila, Maria, Seidel, Kerstin, Holmes, Scott, Wu, Ping, Richman, Joy, Heikinheimo, Kristiin, Chuong, Cheng-Mi, Arnold, Katrin, Hochedlinger, Konrad, Klein, Ophir, Michon, Frederic, Thesleff, Irma Development, 2013;140(7):1424-32. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded BEND6 is a nuclear antagonist of Notch signaling during self-renewal of neural stem cells. Authors: Dai Q, Andreu-Agullo C, Insolera R, Wong L, Shi S, Lai E Development, 2013;140(9):1892-902. Species: Mouse Sample Type: Whole Tissue Application: IHC SOX2 expression associates with stem cell state in human ovarian carcinoma. Authors: Bareiss, Petra M, Paczulla, Anna, Wang, Hui, Schairer, Rebekka, Wiehr, Stefan, Kohlhofer, Ursula, Rothfuss, Oliver C, Fischer, Anna, Perner, Sven, Staebler, Annette, Wallwiener, Diethelm, Fend, Falko, Fehm, Tanja, Pichler, Bernd, Kanz, Lothar, Quintanilla-Martinez, Leticia, Schulze-Osthoff, Klaus, Essmann, Frank, Lengerke, Claudia Cancer Res, 2013;73(17):5544-55. Species: Human Sample Type: tissue Application: IHC - Not specified Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes. Authors: Engelen E, Akinci U, Bryne JC Nat. Genet., 2011;43(6):607-11. Species: Human Sample Type: Cell Lysates Application: ChIP Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord. Authors: Belgacem YH, Borodinsky LN, Proc. Natl. Acad. Sci. U.S.A., 2011;108(11):4482-7. Species: Xenopus Sample Type: Whole Tissue Application: IHC Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours. Authors: Rijlaarsdam MA, van Herk HA, Gillis AJ Br. J. Cancer, 2011;105(6):854-63. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells. Authors: Chng Z, Teo A, Pedersen RA, Vallier L Cell Stem Cell, 2010;6(1):59-70. Species: Human Sample Type: Cell Lysates Application: ChIP A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Authors: Klim JR, Li L, Wrighton PJ Nat. Methods, 2010;7(12):989-94. Species: Human Sample Type: Whole Cells Application: ICC Activity-dependent expression of Lmx1b regulates specification of serotonergic neurons modulating swimming behavior. Authors: Demarque M, Spitzer NC Neuron, 2010;67(2):321-34. Species: Xenopus Sample Type: Whole Tissue Application: IHC Frozen Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Authors: Pollard SM, Yoshikawa K, Clarke ID, Danovi D, Stricker S, Russell R, Bayani J, Head R, Lee M, Bernstein M, Squire JA, Smith A, Dirks P Cell Stem Cell, 2009;4(6):568-80. Species: Human Sample Type: Whole Cells Application: ICC Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells. Authors: Lee J, Son MJ, Woolard K, Donin NM, Li A, Cheng CH, Kotliarova S, Kotliarov Y, Walling J, Ahn S, Kim M, Totonchy M, Cusack T, Ene C, Ma H, Su Q, Zenklusen JC, Zhang W, Maric D, Fine HA Cancer Cell, 2008;13(1):69-80. Species: Human Sample Type: Whole Cells Application: ICC Hyaluronan-CD44 interaction activates stem cell marker Nanog, Stat-3-mediated MDR1 gene expression, and ankyrin-regulated multidrug efflux in breast and ovarian tumor cells. Authors: Bourguignon LY, Peyrollier K, Xia W, Gilad E J. Biol. Chem., 2008;283(25):17635-51. Species: Human Sample Type: Cell Lysates Application: WB Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Authors: Fong H, Hohenstein KA, Donovan PJ Stem Cells, 2008;26(8):1931-8. Species: Human Sample Type: Whole Cells Application: ICC New cell lines from mouse epiblast share defining features with human embryonic stem cells. Authors: Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD Nature, 2007;448(7150):196-9. Species: Mouse Sample Type: Whole Cells Application: ICC Characterization of progenitor domains in the developing mouse thalamus. Authors: Vue TY, Aaker J, Taniguchi A J. Comp. Neurol., 2007;505(1):73-91. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Microglia instruct subventricular zone neurogenesis. Authors: Walton NM, Sutter BM, Laywell ED, Levkoff LH, Kearns SM, Marshall GP, Scheffler B, Steindler DA Glia, 2006;54(8):815-25. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human SOX2 Gly135-Met317 Accession # P48431
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-5).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Graham, V. et al. (2003) Neuron 39:749. Avilion, A.A. et al. (2003) Genes Dev. 17:126. Kishi, M. et al. (2000) Development 127:791. Yuan, H. et al. (1995) Genes Dev. 9:2635. Uwanogho, D. et al. (1995) Mech. Dev. 49:23. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

参考图片

Detection of Human SOX2 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SOX2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of SOX2-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.

SOX2 in Mouse Cortical Stem Cells. SOX2 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected in stem cells using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and co-stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

SOX2 in Rat Cortical Stem Cells. SOX2 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of Human SOX2 by Simple Western^TM. Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 53 kDa (as indicated) using 5 µg/mL of Goat Anti-Human SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

SOX2 in ADLF1 and FAB2 Stem Cell Lines. SOX2 was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.