Human ROR1 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human ROR1 Affinity Purified Polyclonal Ab (100 UG)

货号： AF2000 基本售价： 5076.2 元规格： -

产品信息

概述
货号	AF2000
别名	EC 2.7.10.1; MGC99659; neurotrophic tyrosine kinase receptor-related 1; Neurotrophic tyrosine kinase, receptor-related 1; NTRKR1; NTRKR1dJ537F10.1; receptor tyrosine kinase-like orphan receptor 1; tyrosine-protein kinase transmembrane receptor ROR1
全称	Receptor Tyrosine Kinase-like Orphan Receptor 1
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human ROR1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human ROR2 is observed.
使用方法	Western Blot: 1 µg/mL Simple Western: 25 µg/mL Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4919 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Ror1 is a pseudokinase that is crucial for met-driven tumorigenesis. Authors: Gentile A, Lazzari L, Benvenuti S, Trusolino L, Comoglio PM Cancer Res., 2011;71(8):3132-41. Species: Human Sample Type: Cell Lysates Application: IP The B-cell tumor-associated antigen ROR1 can be targeted with T cells modified to express a ROR1-specific chimeric antigen receptor. Authors: Hudecek M, Schmitt TM, Baskar S Blood, 2010;116(22):4532-41. Species: Human Sample Type: Whole Cells Application: Flow Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia. Authors: Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C Clin. Cancer Res., 2008;14(2):396-404. Species: Human Sample Type: Whole Cells Application: Flow Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion. Authors: Henry C, Llamosas E, Knipprath-Meszaros A, Schoetzau A, Obermann E, Fuenfschilling M, Caduff R, Fink D, Hacker N, Ward R, Heinzelmann-Schwarz V, Ford C Oncotarget, 0;6(37):40310-26. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human ROR1 Ala24-Glu403 Accession # Q01973
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ROR1 belongs to the ROR family of receptor tyrosine kinases, which arecharacterized by: the extracellular immunoglobulin domain,cysteine-rich domain that resembles the Wnt-binding sites of Frizzledproteins, kringle domain and intracellular kinase domain. Human ROR1shares 97% and 58% amino acid sequence identity with mouse ROR1 andhuman ROR2, respectively.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Mini-review: Wnt Receptors & Pathways Receptor Tyrosine Kinases (RTKs) Receptor Tyrosine Kinases (RTKs) in the Akt Pathway Wnt Family Wnt Receptors

参考图片

Detection of ROR1 by Western Blot. Western blot shows lysate of NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ROR1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of ROR1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000, filled histogram) or isotype control antibody (Catalog #
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.