Human/Mouse/Rat HIF-1 alpha Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human/Mouse/Rat HIF-1 alpha Affinity Purified Polyclonal Ab (100 UG)

货号： AF1935 基本售价： 5076.2 元规格： -

产品信息

概述
货号	AF1935
别名	ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF-1 alpha; HIF1A; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1HIF1-ALPHA; PAS domain-containing protein 8; PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
全称	Hypoxia-inducible Transcription Factor 1 alpha
反应种属	Human/Mouse/Rat
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunoprecipitation(2 µg/500 µg cell lysate) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 10⁶cells)
目标/特异性	Detects human, mouse and rat HIF-1 alpha.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunoprecipitation: 2 µg/500 µg cell lysate Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3091 (Human); 15251 (Mouse); 29560 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HEREGULIN/ErbB3 SIGNALING ENHANCES CXCR4-DRIVEN Rac1 ACTIVATION AND BREAST CANCER CELL MOTILITY VIA HIF-1? Mol Cell Biol, 2016;0(0):. Species: Human Sample Type: Chromatin Application: ChIP Assay Identification of ?-Dystrobrevin as a Direct Target of miR-143: Involvement in Early Stages of Neural Differentiation PLoS ONE, 2016;11(5):e0156325. Species: Human Sample Type: Cell Lysates Application: WB High Glucose Up-regulates ADAM17 through HIF-1alpha in Mesangial Cells. Authors: Li R, Uttarwar L, Gao B, Charbonneau M, Shi Y, Chan J, Dubois C, Krepinsky J J Biol Chem, 2015;290(35):21603-14. Species: Rat Sample Type: Cell Lysates Application: ChIP Warburg-like Glycolysis and Lactate Shuttle in Mouse Decidua during Early Pregnancy. Authors: Zuo R, Gu X, Qi Q, Wang T, Zhao X, Liu J, Yang Z J Biol Chem, 2015;290(35):21280-91. Species: Mouse Sample Type: Tissue Homogenates Application: WB Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible factor-1 (HIF-1) in the testis. Authors: Palladino M, Shah A, Tyson R, Horvath J, Dugan C, Karpodinis M Reprod Biol Endocrinol, 2012;10(0):104. Species: Mouse Sample Type: Tissue Homogenates Application: ChIP The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells. Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z BMC Cancer, 2012;12(0):201. Species: Human Sample Type: Cell Lysates Application: WB HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival response of cardiomyocytes to hypoxia. Authors: Robador PA, San Jose G, Rodriguez C, Guadall A, Moreno MU, Beaumont J, Fortuno A, Diez J, Martinez-Gonzalez J, Zalba G Cardiovasc. Res., 2011;92(2):247-55. Species: Human Sample Type: Cell Lysates Application: ChIP Development of an in vitro model of myotube ischemia. Authors: Joshi D, Patel H, Baker DM, Shiwen X, Abraham DJ, Tsui JC Lab. Invest., 2011;91(8):1241-52. Species: Mouse Sample Type: Whole Cells Application: WB HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation. Authors: Bollig-Fischer A, Dziubinski M, Boyer A, Haddad R, Giroux CN, Ethier SP Cancer Res., 2010;70(20):7862-73. Species: Human Sample Type: Cell Lysates Application: WB Hypoxia induces leptin gene expression and secretion in human preadipocytes: differential effects of hypoxia on adipokine expression by preadipocytes. Authors: Wang B, Wood IS, Trayhurn P J. Endocrinol., 2008;198(1):127-34. Species: Human Sample Type: Cell Lysates Application: WB The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis. Authors: Colla S, Tagliaferri S, Morandi F, Lunghi P, Donofrio G, Martorana D, Mancini C, Lazzaretti M, Mazzera L, Ravanetti L, Bonomini S, Ferrari L, Miranda C, Ladetto M, Neri TM, Neri A, Greco A, Mangoni M, Bonati A, Rizzoli V, Giuliani N Blood, 2007;110(13):4464-75. Species: Human Sample Type: Nuclear Extracts Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human HIF-1 alpha Arg575-Asn826 Accession # Q16665.1
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Basic Helix-Loop-Helix (bHLH) Transcription Factors Cancer Stem Cell Transcription Factors Hematopoietic Stem Cell Transcription Factors and Regulators HIF Transcription Factors HIF-1 & Vascular Development ICC / IHC Images: HIF-1 alpha Interactive Pathway: HIF Enhancers Interactive Pathway: HIF Repressors Intracellular Factors in Inflammation Intracellular Signaling Molecules in Angiogenesis Notch Pathway ROS and Stress Resistance Transcription Factors and Regulators Transcription Factors in the Akt Pathway Transcriptional Regulators in Allergy and Asthma

参考图片

Detection of Human HIF‑1 alpha by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 150 μM CoCl₂ for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF‑1 alpha Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF‑1 alpha at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of HIF‑1 alpha -regulated Genes by Chromatin Immunoprecipitation. Mouse primary kidney cells treated with 150 μM CoCl2 for overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF‑1 alpha /DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat HIF‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The epo promoter was detected by standard PCR.

Detection of Human HIF‑1 alpha by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O₂), loaded at 0.2 mg/mL. A specific band was detected for HIF‑1 alpha at approximately 115 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HIF‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.