Human SOX17 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human SOX17 Affinity Purified Polyclonal Ab (100 UG)

货号： AF1924 基本售价： 5076.2 元规格： -

产品信息

概述
货号	AF1924
别名	FLJ22252; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3
全称	Transcription Factor SOX17
反应种属	Human
应用	Western Blot(1 µg/mL) Simple Western(10 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX18 is observed.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	64321 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Changes in Parthenogenetic Imprinting Patterns during Reprogramming by Cell Fusion Authors: Hyun Sik Jang PLoS ONE, 2016;11(5):e0156491. Species: Mouse Sample Type: Whole Cells Application: IHC - Not specified The Anterior-Posterior Patterning of Definitive Endoderm Generated from Human Embryonic Stem Cells Depends on the Differential Signaling of Retinoic Acid, Wnt- and BMP-Signaling Authors: Claudia Davenport Stem Cells, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: IHC - PFA fixed Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen Authors: A Carpentier, I Nimgaonkar, V Chu, Y Xia, Z Hu, TJ Liang Stem Cell Res, 2016;16(3):640-650. Species: Human Sample Type: Whole Cells Application: Flow Cytometery Selection and dynamics of embryonic stem cell integration into early mouse embryos. Authors: Alexandrova S, Kalkan T, Humphreys P, Riddell A, Scognamiglio R, Trumpp A, Nichols J Development, 2016;143(1):24-34. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Inappropriate cadherin switching in the mouse epiblast compromises proper signaling between the epiblast and the extraembryonic ectoderm during gastrulation Sci Rep, 2016;6(0):26562. Species: Mouse Sample Type: Whole Cells Application: IHC Not Specified Diverse Functions of Retinoic Acid in Brain Vascular Development J Neurosci, 2016;36(29):7786-801. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells. Authors: Nagaoka M, Kobayashi M, Kawai C, Mallanna S, Duncan S PLoS ONE, 2015;10(8):e0136350. Species: Human Sample Type: Whole Cells Application: IHC - Not specified Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Authors: Paull D, Sevilla A, Zhou H, Hahn A, Kim H, Napolitano C, Tsankov A, Shang L, Krumholz K, Jagadeesan P, Woodard C, Sun B, Vilboux T, Zimmer M, Forero E, Moroziewicz D, Martinez H, Malicdan M, Weiss K, Vensand L, Dusenberry C, Polus H, Sy K, Kahler D, Gahl W, Solomon S, Chang S, Meissner A, Eggan K, Noggle S Nat Methods, 2015;12(9):885-92. Species: Human Sample Type: Whole Cells Application: IHC Not Specified Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis. Authors: Kim M, Horst A, Blinka S, Stamm K, Mahnke D, Schuman J, Gundry R, Tomita-Mitchell A, Lough J PLoS ONE, 2015;10(2):e0118670. Species: Human Sample Type: Whole Cells Application: ICC Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines. Authors: Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J PLoS ONE, 2015;10(1):e0116032. Species: Human Sample Type: Whole Cells Application: IHC - Not specified Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Authors: Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M Nature, 2015;526(7574):564-8. Species: Human Sample Type: Whole Tissue Application: IHC Not Specified Sox17-mediated maintenance of fetal intra-aortic hematopoietic cell clusters. Authors: Nobuhisa I, Osawa M, Uemura M, Kishikawa Y, Anani M, Harada K, Takagi H, Saito K, Kanai-Azuma M, Kanai Y, Iwama A, Taga T Mol Cell Biol, 2014;34(11):1976-90. Species: Mouse Sample Type: Embryo Application: IHC OCT-embedded miR-373 is regulated by TGFbeta signaling and promotes mesendoderm differentiation in human Embryonic Stem Cells. Authors: Rosa A, Papaioannou M, Krzyspiak J, Brivanlou A Dev Biol, 2014;391(1):81-8. Species: Human Sample Type: Whole Cells Application: ICC Generation and characterization of induced pluripotent stem cells from Aid-deficient mice. Authors: Shimamoto R, Amano N, Ichisaka T, Watanabe A, Yamanaka S, Okita K PLoS ONE, 2014;9(4):e94735. Species: Mouse Sample Type: Whole Cells Application: ICC Unfolded protein response is required for the definitive endodermal specification of mouse embryonic stem cells via Smad2 and beta-catenin signaling. Authors: Xu H, Tsang K, Wang Y, Chan J, Xu G, Gao W J Biol Chem, 2014;289(38):26290-301. Species: Mouse Sample Type: Whole Cells Application: IHC Not Specified BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo. Authors: Graham, Sarah J, Wicher, Krzyszto, Jedrusik, Agnieszk, Guo, Guoji, Herath, Wishva, Robson, Paul, Zernicka-Goetz, Magdalen Nat Commun, 2014;5(0):5667. Species: Mouse Sample Type: Whole Cells Application: ICC Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells. Authors: Singh A, Chappell J, Trost R, Lin L, Wang T, Tang J, Matlock B, Weller K, Wu H, Zhao S, Jin P, Dalton S Stem Cell Reports, 2013;1(6):532-44. Species: Human Sample Type: Whole Cells Application: ICC Defined extracellular matrix components are necessary for definitive endoderm induction. Authors: Taylor-Weiner H, Schwarzbauer J, Engler A Stem Cells, 2013;31(10):2084-94. Species: Mouse Sample Type: Cell Lysates Application: WB Production of hepatocyte-like cells from human pluripotent stem cells. Authors: Hannan, Nicholas, Segeritz, Charis-P, Touboul, Thomas, Vallier, Ludovic Nat Protoc, 2013;8(2):430-7. Species: Human Sample Type: Whole Cells Application: ICC The endothelial cell line bEnd.3 maintains human pluripotent stem cells. Authors: Joubin, Katherin, Richardson, Amelia, Novoa, Natalia, Tu, Edmund, Tomishima, Mark J Stem Cells Dev, 2012;21(12):2312-21. Species: Human Sample Type: Whole Cells A molecular signature for purified definitive endoderm guides differentiation and isolation of endoderm from mouse and human embryonic stem cells. Authors: Wang P, McKnight K, Wong D, Rodriguez R, Sugiyama T, Gu X, Ghodasara A, Qu K, Chang H, Kim S Stem Cells Dev, 2012;21(12):2273-87. Species: Mouse Sample Type: Cell Lysates Application: WB Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas Nature, 2012;491(7426):765-8. Species: Mouse Sample Type: whole cells Application: ICC Fluorescence CD166(pos) subpopulation from differentiated human ES and iPS cells support repair of acute lung injury. Authors: Soh, Boon Sen, Zheng, Dahai, Li Yeo, Julie Su, Yang, Henry He, Ng, Shi Yan, Wong, Lan Hion, Zhang, Wencai, Li, Pin, Nichane, Massimo, Asmat, Atasha, Wong, Poo Sing, Wong, Peng Che, Su, Lin Lin, Mantalaris, Sakis A, Lu, Jia, Xian, Wa, McKeon, Frank, Chen, Jianzhu, Lim, Elaine H, Lim, Bing Mol Ther, 2012;20(12):2335-46. Species: Human Sample Type: whole cells Application: Flow CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells. Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H Stem Cells, 2011;29(4):609-17. Species: Human Sample Type: Whole Cells Application: ICC Early in vitro differentiation of mouse definitive endoderm is not correlated with progressive maturation of nuclear DNA methylation patterns. Authors: Tajbakhsh J, Gertych A, Fagg WS PLoS ONE, 2011;6(7):e21861. Species: Mouse Sample Type: Whole Cells Application: ICC A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3. Authors: Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ J. Cell. Sci., 2011;124(0):1992-2000. Species: Human Sample Type: Whole Cells Application: ICC Induction of pluripotent stem cells from human third molar mesenchymal stromal cells. Authors: Oda Y, Yoshimura Y, Ohnishi H J. Biol. Chem., 2010;285(38):29270-8. Species: Human Sample Type: Whole Cells Application: ICC Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo. Authors: Morris SA, Teo RT, Li H, Robson P, Glover DM, Zernicka-Goetz M Proc. Natl. Acad. Sci. U.S.A., 2010;107(14):6364-9. Species: Human Sample Type: Whole Cells Application: ICC SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells. Authors: Chng Z, Teo A, Pedersen RA, Vallier L Cell Stem Cell, 2010;6(1):59-70. Species: Human Sample Type: Whole Cells Application: ICC Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm. Authors: Chayosumrit M, Tuch B, Sidhu K Biomaterials, 2010;31(3):505-14. Species: Human Sample Type: Cell Lysate Application: WB The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers. Authors: Emre N, Vidal JG, Elia J PLoS ONE, 2010;5(8):e12148. Species: Human Sample Type: Whole Cells Application: ICC A role for PDGF signaling in expansion of the extra-embryonic endoderm lineage of the mouse blastocyst. Authors: Artus J, Panthier JJ, Hadjantonakis AK Development, 2010;137(20):3361-72. Species: Mouse Sample Type: Whole Cells Application: ICC Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells. Authors: Sun N, Panetta NJ, Gupta DM, Wilson KD, Lee A, Jia F, Hu S, Cherry AM, Robbins RC, Longaker MT, Wu JC Proc. Natl. Acad. Sci. U.S.A., 2009;106(37):15720-5. Species: Human Sample Type: Whole Cells Application: ICC Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Authors: Borowiak M, Maehr R, Chen S, Chen AE, Tang W, Fox JL, Schreiber SL, Melton DA Cell Stem Cell, 2009;4(4):348-58. Species: Human Sample Type: Whole Cells Application: Flow Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells. Authors: Zhang D, Jiang W, Liu M, Sui X, Yin X, Chen S, Shi Y, Deng H Cell Res., 2009;19(4):429-38. Species: Human Sample Type: Whole Cells Application: ICC Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH FEBS Lett., 2009;583(3):561-7. Species: Human Sample Type: Whole Cells Application: ICC Biphasic induction of Pdx1 in mouse and human embryonic stem cells can mimic development of pancreatic beta-cells. Authors: Bernardo AS, Cho CH, Mason S, Docherty HM, Pedersen RA, Vallier L, Docherty K Stem Cells, 2009;27(2):341-51. Species: Human Sample Type: Whole Cells Application: ICC A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Authors: Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P Dev. Biol., 2009;330(2):286-304. Species: Mouse Sample Type: Whole Cells Application: ICC High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Authors: Desbordes SC, Placantonakis DG, Ciro A, Socci ND, Lee G, Djaballah H, Studer L Cell Stem Cell, 2008;2(6):602-12. Species: Human Sample Type: Whole Cells Application: ICC Prospective isolation and global gene expression analysis of definitive and visceral endoderm. Authors: Sherwood RI, Jitianu C, Cleaver O, Shaywitz DA, Lamenzo JO, Chen AE, Golub TR, Melton DA Dev. Biol., 2007;304(2):541-55. Species: Mouse Sample Type: Whole Tissue Application: IHC Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Authors: Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S Proc. Natl. Acad. Sci. U.S.A., 2006;103(18):6907-12. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human SOX17 Asp177-Val414 Accession # Q9H6I2
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Kanai-Azuma, M. et al. (2002) Development 129:2367. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.

参考图片

Detection of Human SOX17 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of SOX17-regulated Genes by Chromatin Immunoprecipitation. Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.

SOX17 in B16 Mouse Cell Line. SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX17 in Human BG01V Cells. SOX17 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human SOX17 by Simple Western^TM. Simple Western lane view shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human SOX17 by Simple Western^TM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.