| 货号 | AF8520 |
| 别名 | FLJ00245; GLBA; proactivator polypeptide; prosaposin; SAP1; SAP1MGC110993; sphingolipid activator protein-1 | 全称 | Prosaposin |
| 反应种属 | Human |
| 应用 | Western Blot(1:1000 dilution) Immunocytochemistry(1:100 dilution) |
| 目标/特异性 | Detects human PSAP in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1:1000 dilution Immunocytochemistry: 1:100 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5660 (Human); 19156 (Mouse); 25524 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf21-derived recombinant human PSAP Gly17-Asn524 Accession # P07602 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 背景 | Prosaposin behaves as a myelinotrophic and neurotrophic factor, whose effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.By similarity Saposins are specific low-molecular mass non-enzymic proteins and participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases. The Prosaposin gets cleaved into 5 chains, Saposin A, B, B-Val, C and D. It also contains several disulfide bonds as well as glycosylation sites. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human PSAP by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human PSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8520) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for PSAP at approximately 60-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
PSAP in A431 Human Cell Line. PSAP was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human PSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8520) at 1:100 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to Golgi. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
