Rat CD34 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Rat CD34 Affinity Purified Polyclonal Ab (100 UG)

货号： AF4117 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF4117
别名	CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1
反应种属	Rat
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects rat CD34 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant porcine CD34 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Systemic siRNA Nanoparticle-Based Drugs Combined with Radiofrequency Ablation for Cancer Therapy. Authors: Ahmed M, Kumar G, Navarro G, Wang Y, Gourevitch S, Moussa M, Rozenblum N, Levchenko T, Galun E, Torchilin V, Goldberg S PLoS ONE, 2015;10(7):e0128910. Species: Rat Sample Type: Whole Tissue Application: IHC Not Specified Renal telocytes contribute to the repair of ischemically injured renal tubules. Authors: Li L, Lin M, Li L, Wang R, Zhang C, Qi G, Xu M, Rong R, Zhu T J Cell Mol Med, 2014;18(6):1144-56. Species: Rat Sample Type: Whole Cells Application: IF Rat hair follicle-constituting cells labeled by a newly-developed somatic stem cell-recognizing antibody: a possible marker of hair follicle development. Authors: Ichikawa C, Izawa T, Juniantito V, Tanaka M, Hori M, Tanaka K, Takenaka S, Kuwamura M, Yamate J Histol Histopathol, 2013;28(2):257-68. Species: Rat Sample Type: tissue Application: IHC Frozen In vivo adipogenesis in rats measured by cell kinetics in adipocytes and plastic-adherent stroma-vascular cells in response to high-fat diet and thiazolidinedione. Authors: Tchoukalova YD, Fitch M, Rogers PM, Covington JD, Henagan TM, Ye J, Hellerstein MK, Ravussin E Diabetes, 2012;61(1):137-44. Species: Rat Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant rat CD34 Glu37-Ile293 Accession # NP_001100672
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Rat CD34 is a 80-100 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352 amino acid (aa) protein with a 257 aa extracellular domain (ECD) (aa 35-291) and 74 aa cytoplasmic tail (aa 313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Acute Myeloid Leukemia B Cell Markers Cancer Stem Cell Markers Cardiac Stem Cell Markers Endothelial Cell Differentiation Markers Endothelial Cell Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Endothelial Progenitor and Endothelial Cell Markers Endothelial Progenitor Cell Markers Group 1 ILCs Hemangioblasts Hematopoietic Progenitor Cell Markers Hematopoietic Stem Cell Markers Human CD Marker Products ICC / IHC Images: CD34 Leukocyte Receptors/Markers in Allergy and Asthma Lymphoid Lineage Markers Muscle Stem Cell Markers Myeloid Lineage Markers Myeloid-derived Suppressor Cells (MDSC) Myogenesis Markers Myogenic Precursor Markers Regulatory T Cells (Tregs) Skin Stem Cell Markers

参考图片

CD34 in Rat Liver. CD34 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Rat CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4117) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.