Rat Nestin Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Rat Nestin Affinity Purified Polyclonal Ab (100 UG)

货号： AF2736 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF2736
别名	FLJ21841; NES; nestin
反应种属	Rat
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10763 (Human); 18008 (Mouse); 25491 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesis. Authors: Gregorian C, Nakashima J, Le Belle J, Ohab J, Kim R, Liu A, Smith KB, Groszer M, Garcia AD, Sofroniew MV, Carmichael ST, Kornblum HI, Liu X, Wu H J. Neurosci., 2009;29(6):1874-86. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Nestin-positive microglia in adult rat cerebral cortex. Authors: Takamori Y, Mori T, Wakabayashi T, Nagasaka Y, Matsuzaki T, Yamada H Brain Res., 2009;1270(0):10-8. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Mechanisms of murine lacrimal gland repair after experimentally induced inflammation. Authors: Zoukhri D, Fix A, Alroy J, Kublin CL Invest. Ophthalmol. Vis. Sci., 2008;49(10):4399-406. Species: Mouse Sample Type: Tissue Homogenates Application: WB Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking. Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD Biotechnol. Bioeng., 2007;97(6):1617-25. Species: Human Sample Type: Cell Lysates Application: WB Vasopressin mediates mitogenic responses to adrenalectomy in the rat anterior pituitary. Authors: Subburaju S, Aguilera G Endocrinology, 2007;148(7):3102-10. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant rat Nestin Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys) Accession # EDM00749
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Nestin is a 240 kDa class VI intermediate filament protein that was first identified in neuroepithelial stem cells (1, 2). It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% amino acid sequence homology with the mouse protein.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310. Lendahl, U. et al. (1990) Cell 60:585.

参考图片

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

beta ‑III Tubulin and Nestin in Rat Cortical Stem Cells. beta ‑III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta ‑III Tubulin Monoclonal (clone TuJ‑1) Antibody (Catalog # MAB1195) and 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.