Canine VEGF 164 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Canine VEGF 164 Affinity Purified Polyclonal Ab (100 UG)

货号： AF1603 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF1603
别名	MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
全称	Vascular Endothelial Growth Factor
反应种属	Canine
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects canine VEGF₁₆₄ in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human VEGF, recombinant mouse VEGF, and recombinant rat VEGF is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize VEGF-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.04-0.10 µg/mL in the presence of 15 ng/mL Recombinant Canine VEGF.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Evaluation of a xenogeneic VEGF vaccine in dogs with soft tissue sarcoma. Authors: Kamstock D, Elmslie R, Thamm D Cancer Immunol. Immunother., 2007;56(8):1299-309. Species: Canine Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant canine VEGF₁₆₄ Pro28-Arg190 Accession # NP_001103972
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Canine
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Adipocytokines Adult Neurogenesis Airway Inflammation and Remodeling ALS Related Molecules BIObrief: Neuroprotective & Pathogenic Effects of VEGF Signaling Cancer Biomarkers Developmental Factors Implicated in Neurodegeneration HIF Transcription Factors HIF-1 & Vascular Development ICC / IHC Images: VEGF Inflammatory Mediators Macrophage Activation Markers Mini-review: Vascular Endothelial Growth Factor (VEGF) Myeloid-derived Suppressor Cells (MDSC) New and Improved TNF Superfamily Proteins 2015 Platelets Preeclampsia Proliferation Bioassay HUVECs Regulation of VE-Cadherin and VEGF R2 by VEGF Technical Notes: Serum Levels of Vascular Growth Factors During Pregnancy VEGF & Amyotrophic Lateral Sclerosis VEGF Family VEGF Family Ligands VEGF, Angiopoietin-2 and Tumor Vascularization

参考图片

Cell Proliferation Induced by VEGF and Neutralization by Canine VEGF Antibody. Recombinant Canine VEGF (Catalog # 1603-CV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine VEGF (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine VEGF₁₆₄ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603). The ND₅₀ is typically 0.04‑0.10 µg/mL.