| 货号 | AF774 |
| 别名 | CD102 antigen; CD102; ICAM-2; intercellular adhesion molecule 2 | 全称 | Intercellular Adhesion Molecule 2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.25 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse ICAM-2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human ICAM‑2 and recombinant rat ICAM-1 and less than 1% cross-reactivity with recombinant mouse (rm) VCAM-1, rmICAM-5, and rmICAM-1 is observed. |
| 使用方法 | Western Blot: 0.25 µg/mL Immunohistochemistry: 5-15 µg/mL Adhesion Blockade: The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Mouse ICAM-2/CD102 Fc Chimera (Catalog # 774-M2, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3384 (Human); 15896 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse ICAM-2 Ser20-Gln222 Accession # P35330 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Intercellular Adhesion Molecule-2 (ICAM-2, also known as CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration. The mouse and human ICAM-2 proteins share 60% amino acid identity. Human LFA-1 binds equally well to human or mouse ICAM-2. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Mouse ICAM‑2/CD102 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ICAM‑2/CD102 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF774) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for ICAM‑2/CD102 at approximately 50 to 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
ICAM‑2/CD102 in Mouse Ovary. ICAM‑2/CD102 was detected in perfusion fixed frozen sections of mouse ovary using 15 µg/mL Goat Anti-Mouse ICAM‑2/CD102 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF774) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
