Mouse TCF-3/E2A Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Mouse TCF-3/E2A Affinity Purified Polyclonal Ab (100 UG)

货号： AF7650 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF7650
别名	bHLHb21; bHLHb21TCF-3; Class B basic helix-loop-helix protein 21; E2A immunoglobulin enhancer-binding factor E12/E47; E2A; E2AKappa-E2-binding factor; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; ITF1Transcription factor ITF-1; Kappa-E2-binding factor; MGC129647; MGC129648; Pan2; Tcfe2a; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor E2-alpha; VDIR; VDR interacting repressor
全称	Transcription Factor 3/E2-alpha
反应种属	Mouse
应用	Western Blot(0.5 µg/mL)
目标/特异性	Detects mouse TCF-3/E2A in direct ELISAs and Western blots.
使用方法	Western Blot: 0.5 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6929 (Human); 21423 (Mouse); 312451 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse TCF-3/E2A Asn33-Arg159 Accession # P15806
生物活性	Mouse
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	TCF-3 (Transcription factor 3; also Transcription factor A1 and E2A/alpha plus E12/E47) is a 68-75 kDa nuclear class I member of the bHLH family of molecules. It should not be confused with TCF-3, otherwise known as TCF7-L1, an epidermis-associated beta -catenin regulator. TCF-3 is ubiquitously expressed, and generally serves as a transcriptional regulator. It is able to form covalent homodimers, and following phosphorylation, heterodimers with tissue-specific class II bHLH factors, thus impacting the proliferation and differentiation of select cell types. While heterodimers, and possibly homodimers, activate genes by binding to E boxes, heterodimers involving Id proteins are gene repressors. Mouse TCF-3/E12 is 651 amino acids (aa) in length. It contains one Leu-zipper motif (aa 387-422) plus a bHLH domain (aa 544‑604). TCF-3 contains at least three potential Ser phosphorylation sites. There is one alternative splice form that is termed E47 and shows a 69 aa substitution for aa 527-598. This involves the bHLH domain, and it is believed that each splice form has a distinct function(s). Both splice forms (E12 and E47) do occur simultaneously in the same cell type. Over aa 33-159, mouse TCF-3 shares 94% and 75% aa sequence identity with rat and human TCF-3, respectively.
运输条件	Blue Ice
存放说明	-20℃
参考文献	B Cell Development and Differentiation Cancer Biomarkers Epithelial Stem Cell Transcription Factors HIF Transcription Factors Homeodomain (Hox) Transcription Factors ICC / IHC Images: TCF-3/E2A Neural Stem Cell Transcription Factors Pluripotent Stem Cell Transcription Factors T Cell Antigen Recognition Wnt Intracellular Signaling

参考图片

Detection of Mouse TCF‑3/E2A by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, P19 mouse embryonal carcinoma cell line, C2C12 mouse myoblast cell line, and beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse TCF‑3/E2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7650) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for TCF‑3/E2A at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.