Mouse IGSF8/CD316 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Mouse IGSF8/CD316 Affinity Purified Polyclonal Ab (100 UG)

货号： AF3117 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF3117
别名	CD316 antigen; CD316; CD81P3; CD81P3LIR-D1; EWI2; EWI-2; EWI2CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; IgSF8; immunoglobulin superfamily, member 8; KCT4; KCT-4; Keratinocytes-associated transmembrane protein 4; PGRL; PGRLimmunoglobulin superfamily member 8
全称	Immunoglobulin Superfamily, Member 8
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse IGSF8/CD316 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	93185 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Proc Natl Acad Sci U S A, 2015;112(15):E1946-55. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified EWI-2 negatively regulates TGF-beta signaling leading to altered melanoma growth and metastasis. Authors: Wang H, Sharma C, Knoblich K, Granter S, Hemler M Cell Res, 2015;25(3):370-85. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified IgSF8: a developmentally and functionally regulated cell adhesion molecule in olfactory sensory neuron axons and synapses. Authors: Ray A, Treloar H Mol Cell Neurosci, 2012;50(3):238-49. Species: Mouse Sample Type: Cell Lysates Application: IP
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IGSF8/CD316 Ala25-Thr577 Accession # NP_536344
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IGSF8, also known as PGRL (PG Regulatory-Like Protein), KASP (KAI/CD82 Associated Protein) and EWI-2 (Glu-Trp-Ile motif 2), is a widely expressed transmembrane adhesion protein. It interacts with beta 1 Integrins and various tetraspanins including CD9, CD81 and CD82. IGSF8 contains four extracellular Ig-like domains. IGSF8 overexpression in transformed cells inhibits cell migration and suppresses cancer metastatic potential. Mouse and human IGSF8 share 90% amino acid sequence identity.
运输条件	Blue Ice
存放说明	-20℃
参考文献	B Cell Chemotaxis, Migration, and Adhesion Integrins and Associated Molecules T Cell Migration/Adhesion Thymocytes

参考图片

Detection of IGSF8/CD316 in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro‑2A mouse neuroblastoma cell line was stained with Goat Anti-Mouse IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

IGSF8/CD316 in Neuro‑2A Mouse Cell Line. IGSF8/CD316 was detected in immersion fixed Neuro‑2A mouse neuroblastoma cell line using Goat Anti-Mouse IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.