| 货号 | AF2346 |
| 别名 | CWCV, and Kazal-like domains proteoglycan 3; HSAJ1454; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3; SPOCK3; TES-3; testican 3; TICN3 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects mouse Testican 3/SPOCK3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross‑reactivity with recombinant human (rh) Testican 3 is observed, less than 5% cross‑reactivity with rhTestican 1 is observed, and less than 1% cross-reactivity with rhTestican 2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 50859 (Human); 72902 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Testican 3/SPOCK3 Ala26-Ile436 Accession # Q8BKV0 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Testican 3 encoded by the SPOCK3 gene is a proteoglycan expressed in brain (1). The human and mouse cDNAs predict 90% identity between the deduced amino acid sequences from the two species, indicating a conserved function (2, 3). Only the human protein, but not the mouse protein, has been characterized in the literature. Testican 3 contains Ca2+-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to testicans, is responsible for the inhibition of Testican 3 towards MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (1). The thyropin domain and the follistatin-like domain with a six cysteine Kazal-like motif may inhibit cysteine and serine proteases, respectively (4). A spliced variant designated as N-Tes contains the N-terminal unique region, the follistatin-like domain and the Ca2+-binding domain, but lacks the C-terminal thyropin domain and the acidic domain (1). The purified rmTestican 3 is capable of inhibiting rhMMP-14 and rhCathepsin L (R&D Systems, Catalog # 918-MP and 952-CY) in assays using the fluorogenic peptide substrates (R&D Systems, Catalog # ES001 and ES008). As compared to rhTestican 1 (R&D Systems, Catalog # 2327-PI), the IC50 of rmTestican 3 is weaker toward rhCathepsin L and rhMMP-14 activity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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