货号 | AF1937 |
别名 | MCP-7; Mcpt7; TPSB1 |
反应种属 | Mouse |
应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) |
目标/特异性 | Detects mouse Tryptase beta ‑1/MCP‑7/Mcpt7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) TPSB2 is observed, 5% cross-reactivity with rhTPS1 is observed, and less than 1% cross-reactivity with rhTPSG1 and recombinant mouse MCPT1 is observed. |
使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 17230 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Epidermal RAF prevents allergic skin disease | |
纯化方式 | Antigen Affinity-purified |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Tryptase beta ‑1/MCP‑7/Mcpt7 Ile29-Phe273 Accession # Q02844 |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | Tryptase beta -1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid sequence for mouse Tryptase beta -1 consists of a signal peptide (residues 1‑18), a pro region (residues 19‑28) and the mature chain (residue 29‑273). The mature chain was expressed and purified. |
运输条件 | Blue Ice |
存放说明 | -20℃ |
参考文献 |
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