Human/Mouse STAT1 p91 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human/Mouse STAT1 p91 Affinity Purified Polyclonal Ab (100 UG)

货号： PAF-ST1 基本售价： 4996.9 元规格： -

产品信息

概述
货号	PAF-ST1
别名	CANDF7; DKFZp686B04100; ISGF-3; ISGF-3,91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT91
全称	Signal Transducer and Activator of Transcription 1
反应种属	Human/Mouse
应用	Western Blot(1 µg/mL) Immunoprecipitation(3 µg/10⁶cells)
目标/特异性	Detects human and mouse STAT1 p91 in Western blots. In Western blots, less than 1% cross-reactivity with human or mouse STAT1 p84 is observed. Additionally, no cross-reactivity with the other STATs has been detected by immunoprecipitation or Western blotting.
使用方法	Western Blot: 1 µg/mL Immunoprecipitation: 3 µg/10⁶cells
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6772 (Human); 20846 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human STAT1
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	STAT1 is associated with type I and II interferon signaling. Phosphorylation of STAT1 at Y701 leads to dimerization and translocation to the nucleus to activate gene transcription. Human STAT1 shows 93% and 94% aa identity with mouse and rat STAT1, respectively, over the region used as an immunogen. This region is identical between isoforms STAT1a (91 kDa) and STAT1b (84 kDa).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Apoptosis Transcription Factors and Regulators B Cell Markers Chemokine Receptor Intracellular Signaling Common gamma Chain Receptor Family IL-10/Interferon Family IL-12 Family Immunoglobulin-Like Domain Transcription Factors Interactive Pathway: Th1 Differentiation Intracellular Signaling Molecules in Angiogenesis Jak and STAT Proteins Macrophage Activation Markers Mesenchymal Stem Cell Transcription Factors Mini-review: STAT Proteins Myeloid-derived Suppressor Cells (MDSC) PDGF Family STAT Th1 Cells Transcription Factors in the Akt Pathway Transcriptional Regulators in Allergy and Asthma

参考图片

Detection of Human and Mouse STAT1 p91 by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for STAT1 p91 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunoprecipitation of Human STAT1. STAT1 p91 was immunoprecipitated from 200 μg of cell lysate of HeLa human cervical epithelial carcinoma cell line following incubation with 3 µg Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) overnight at 4 °C. STAT1 p91-antibody complexes were absorbed using Protein G sepharose (Invitrogen, Catalog # 10-1242). Immunoprecipitated STAT1 p91 was detected by Western blot using 1 µg/mL Human STAT1 Monoclonal Antibody (Catalog # MAB14901). View our recommended buffer recipes for immunoprecipitation.