| 货号 | AF969 |
| 别名 | FGF-19; fibroblast growth factor 19 | 全称 | Fibroblast Growth Factor 19 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human FGF-19 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, recombinant mouse (rm) FGF-8b, rmFGF-8c, rhFGF-9, rhFGF-10, rmFGF-15, rhFGF-17, and rhFGF-18 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 2-8 µg/mL of this antibody will block 50% of the binding of 250 ng/mL of Recombinant Human FGF-19 (Catalog # 969-FG) to immobilized Recombinant Human FGF R4 Fc Chimera (Catalog # 685-FR) coated at 2 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9965 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterization of a FGF19 variant with altered receptor specificity revealed a central role for FGFR1c in the regulation of glucose metabolism. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FGF-19 Phe27-Lys216 Accession # O95750 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4). Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites (1, 2). Among FGF family members, human FGF-19 is most closely related to chicken FGF-19 and murine FGF-15, sharing approximately 61% and 51% aa sequence identity, respectively (1, 2, 5). Neither the human orthologue of mouse FGF-15, nor the mouse counterpart of human FGF-19 has been identified. With the exception of adult gall bladder epithelium, FGF-19 expression is restricted to fetal tissues (1, 2). Unlike most FGFs which bind to and activate more than one FGF receptor, FGF-19 is a specific ligand for FGF R4 (2). Similarly, another FGF family member, FGF-7 (KGF), only activates KGF R, the IIIb isoform of FGF R2 (4). During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development (5). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human FGF‑19 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, conditioned media from COLO 205 cell line, and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human FGF‑19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FGF‑19 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human FGF‑19 by Simple WesternTM. Simple Western lane view shows conditioned media from COLO 205 human colorectal adenocarcinoma cell line and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FGF‑19 at approximately 26 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FGF‑19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system. |
