Human MMP-14/MT1-MMP Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human MMP-14/MT1-MMP Affinity Purified Polyclonal Ab (100 UG)

货号： AF918 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF918
别名	EC 3.4.24; EC 3.4.24.80; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1
全称	Matrix Metalloproteinase 14/Membrane Type 1 MMP
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, -12, -13, -15, and -16 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4323 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Site-Specific Expression of Gelatinolytic Activity during Morphogenesis of the Secondary Palate in the Mouse Embryo. Authors: Gkantidis, Nikolaos, Blumer, Susan, Katsaros, Christos, Graf, Daniel, Chiquet, Matthias PLoS ONE, 2012;7(10):e47762. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Tumor cell invasion of collagen matrices requires coordinate lipid agonist-induced G-protein and membrane-type matrix metalloproteinase-1-dependent signaling. Authors: Fisher KE, Pop A, Koh W Mol. Cancer, 2006;5(0):69. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human MMP‑14/MT1‑MMP
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha _V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (2). Recombinant Human MMP-14 consists of the pro and catalytic domains.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Seike, M. (2003) Cancer Lett. 194:1. Sato, H. et al. (1994) Nature 370:61.

参考图片

Detection of MMP-14/MT1-MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Goat Anti-Human MMP‑14/MT1‑MMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF918, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).