| 货号 | AF908 |
| 别名 | CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL | 全称 | Matrix Metalloproteinase 8 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human MMP-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) MMP-8 and recombinant rat MMP-8 is observed, and less than 1% cross-reactivity with recombinant human (rh) MMP-1, rhMMP-2, rhMMP-3, rhMMP-7, rhMMP-9, rhMMP-10, rhMMP-12, rhMMP-13, rmMMP-3, and rmMMP-9 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4317 (Human); 17394 (Mouse); 63849 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Matrix-metalloproteinase-14 deficiency in bone-marrow-derived cells promotes collagen accumulation in mouse atherosclerotic plaques. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human MMP‑8 Phe21-Gly467 Accession # AAZ38714 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP‑8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP‑8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP-8 is heavily glycosylated. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
MMP‑8 in Human Breast. MMP‑8 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human MMP‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF908) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
MMP‑8 in Jurkat Human Cell Line. MMP‑8 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human MMP‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF908) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
