Human Mer Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human Mer Affinity Purified Polyclonal Ab (100 UG)

货号： AF891 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF891
别名	c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; MER; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer
全称	Receptor Tyrosine Protein Kinase Mer
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Mer and less than 1% cross-reactivity with recombinant human (rh) Axl and rhDtk is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10461 (Human); 17289 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation Proc Natl Acad Sci USA, 2016;0(0):. Species: Human Sample Type: Cell Culture Supernates Application: WB Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012;209(11):2033-47. Species: Human Sample Type: Cell Lysates Application: WB Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. Authors: Zizzo G, Hilliard B, Monestier M, Cohen P J Immunol, 2012;189(7):3508-20. Species: Human Sample Type: Whole Cells Application: Neut Ectosomes released by polymorphonuclear neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages. Authors: Eken C, Martin PJ, Sadallah S, Treves S, Schaller M, Schifferli JA J. Biol. Chem., 2010;285(51):39914-21. Species: Human Sample Type: Whole Cells Application: Neut Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells. Authors: Uehara H, Shacter E J. Immunol., 2008;180(4):2522-30. Species: Human Sample Type: Cell Lysates Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Mer Arg26-Ala499 Accession # Q12866
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Nagata, K.et al. (1996) J. Biol. Chem. 22:30022. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

参考图片

Detection of Human Mer by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and PANC‑1 human pancreatic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Mer at approximately 170-230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.