| 货号 | AF7890 |
| 别名 | 1300008B03Rik; 2310031E04Rik; HMFT1766; leucine-rich alpha-2-glycoprotein 1; leucine-rich alpha-2-glycoprotein; LRGleucine rich alpha 2 glycoprotein | 全称 | Leucine-rich alpha-2-glycoprotein 1 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human LRG1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 116844 (Human); 76905 (Mouse); 367455 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Human embryonic kidney cell line HEK293-derived recombinant human LRG1 Val36-Gln347 Accession # P02750 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LRG-1 (Leucine-Rich alpha-2-Glycoprotein 1; also LRHG [in rodent]) is a 45-55 kDa, monomeric, variably glycosylated, secreted protein member of the leucine-rich repeat family of molecules. It should not be confused with either LRIG-1/ Leucine-Rich repeats and Ig-like domains-1, or LRG1/LIM-RhoGap protein 1. LRG-1 has a limited expression pattern, being associated with neutrophils, neutrophil precursors (myelocytes; metamyelocytes), astrocytes, HEV (in mice), hepatocytes and select tumor cells. LRG-1 is reported to bind multiple molecules, including TGF-beta, extracellular cytochrome C, and fibronectin plus collagen IV of the ECM. Functions associated with this binding include granulocyte maturation, protection from apoptosis, and cell migration. Mature human LRG-1 is 312 amino acids (aa) in length (aa 35-347). Depending upon the source, LRG-1 has been described as having either thirteen 24 aa leucine-rich repeats (LRRs), or eight LRRs spanning aa 93-282 coupled to a C-terminal LRR. Variable O- and N-based glycosylation accounts for the variablility in MW. Mature human LRG-1 shares 66% aa sequence identity with mouse LRG-1. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human LRG1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7890) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRG1 at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
LRG1 in Human Brain. LRG1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7890) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
