| 货号 | AF7687 |
| 别名 | 6-phosphofructokinase, muscle type; EC 2.7.1,6-phosphofructo-1-kinase; EC 2.7.1.11; GSD7; MGC8699; PFK1; PFK-1; PFKA; PFK-A; PFKM; PFKX; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; phosphofructokinase, muscle; phosphofructokinase, polypeptide X; Phosphofructokinase-1; Phosphofructokinase-M; Phosphohexokinase | 全称 | Phosphofructokinase, Muscle |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Phosphofructokinase/PFKM in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5213 (Human); 18642 (Mouse); 65152 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Phosphofructokinase/PFKM Asn674-Val780 Accession # P08237 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | PFKM (PhosphoFructoKinase, Muscle type; also PFK-A and Phosphohexokinase)) is a 85-86 kDa member of the phosphofructokinase family, two domain subfamily of enzymes. It is one of three functionally related enzymes, all expressed by distinct genes. While PFKM is expressed by virtually all cells, its name is based on the fact that skeletal muscle expresses only the PFKM isotype. PFK catalyzes the essentially irreversible phosphorylation of Fru-6-P, forming Fru-1,6-bisP. This determines the rate of cellular glycolysis. As a monomer, PFKM is unstable, and following synthesis, PFKM forms low activity homodimers. High activity comes with tetramerization/oligomerization. In all cases, multimerization is associated with PFKM interaction with various components of the cell cytoskeleton. Human PFKM is 780 amino acids (aa) in length. It contains a 740 aa catalytic region (aa 16-755) plus one utilized phosphorylation site at Thr2. There are at least two potential isoform variants. One contains an alternative start site 71 aa upstream of the standard site, while another shows a deletion of aa 282-312. Proteolysis of PKFM apparently occurs, generating 45-47 kDa fragments. Over aa 674-780, human PFKM shares 98% aa sequence identity with mouse PFKM. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Muscle Phosphofructokinase/PFKM by Western Blot. Western blot shows lysates of human aorta tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Muscle Phosphofructokinase/PFKM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7687) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
