| 货号 | AF7639 |
| 别名 | HC3; MU; PMSA2; PSC3 | 全称 | Proteasome (Prosome, Macropain) Subunit, alpha Type, 2 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat PSMA2 in Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5683 (Human); 19166 (Mouse); 29669 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PSMA2 Ala2-Ala234 Accession # P25787 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | PSMA2 (Proteasome subunit alpha type 2; also proteasome component C3 and Macropain subunit C3) is an intracellular 25-27 kDa member of the peptidase T1A family of molecules. It is ubiquitously expressed, and participates in the formation of the 700 kDa 20S proteasomal complex. This complex contains four stacked rings, forming a barrel. The top and bottom rings are made of seven alpha -subunits, while the two central rings are composed of seven catalytic beta -subunits. The complex degrades 80-90% of all intracellular proteins through both a ubiquitin-dependent and -independent process. PSMA2 may modulate the activity of select beta -subunits. Human PSMA2 is 234 amino acids (aa) in length. It contains at least five utilized phosphorylation sites plus one nitrated tyrosine. Full-length human PSMA2 shares 99% aa sequence identity with mouse PSMA2. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat PSMA2 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat PSMA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7639) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PSMA2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
