| 货号 | AF6959 |
| 别名 | ALC1; amplified in liver cancer 1; amplified in liver cancer protein 1; CHDL; chromodomain helicase DNA binding protein 1-like; chromodomain-helicase-DNA-binding protein 1-like; FLJ22530 | 全称 | Chromodomain Helicase DNA Binding Protein 1-like |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CHD1L in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) CHD1 and rhCHD5 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9557 (Human); 68058 (Mouse); 310707 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human CHD1L Arg759-Lys879 Accession # Q86WJ1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CHD-1L (Chromohelicase/ATPase DNA-binding protein 1-Like; also ALC-1) is a 98-118 kDa member of the SNF2/RAD54 helicase family of proteins. It is expressed in hepatocytes, possesses ATPase activity, and likely promotes chromatin remodeling at sites of DNA damage. It also is considered an oncogene. CHD-1L upregulates ARHGEF9 trabscription, an action that promotes Cdc42 activity with accompanying filopodia formation and EMT. It also binds apoptosis-mediating Nur77, blocking its migration from nucleus-to-mitochondria. Human CHD-1L is 897 amino acids (aa) in length. It contains a helicase ATP-binding domain (aa 58-223), a C-terminal helicase domain (aa 351-513), a coiled-coil region (aa 38-675) and one Macro domain that binds poly-ADP-ribose and targets DNA damage sites (aa 704-897). There are at least two Ser/Thr phosphorylation sites. There are multiple potential splice variants. One isoform contains an alternative start site at Met114, a second isoform possesses a deletion of aa 43-246, a third isoform shows a three aa substitution for aa 363-897, and a fourth isoform combines a 16 aa insertion after Arg386 with a five aa substitution for aa 425-897. Over aa 759-879, human CHD-1L shares 94% aa identity with mouse CHD-1L. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human CHD1L by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CHD1L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6959) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD1L at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
