| 货号 | AF6947 |
| 别名 | BYE1; C20orf158; DATF1; DATF-1; DATF1DIDO3; death associated transcription factor 1; death inducer-obliterator 1; Death-associated transcription factor 1; death-inducer obliterator 1; DIO1; DIO1DIDO2; DIO-1hDido1; dJ885L7.8; DKFZp434P1115; FLJ11265; KIAA0333chromosome 20 open reading frame 158; MGC16140 | 全称 | Death Inducer Obliterator 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects human DIDO1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11083 (Human); 23856 (Mouse); 362286 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DIDO1 Met1-Ser117 Accession # Q9BTC0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | DIDO1 (Death-inducer obliterator 1; also DIO-1, C20orf158 and DATF-1) is a 245 kDa (predicted) intracellular protein that belongs the DIO family of proteins. It is ubiquitous in expression, and apparently serves multiple functions, depending on its splice variant. The standard form is a nuclear protein that maintains the integrity of mitotic checkpoint protein BubR1. An absence of DIDO1 results in centrosome amplification and unequal chromatin segregation. The best known short form (an 83 kDa isoform in mouse), by contrast, induces apoptosis by promoting transcription of caspase 9. Human standard form DIDO1 is 2240 amino acid (aa) in length. It contains two NLSs (aa 165-193), one PHD-type Zn finger region (aa 270-320), a TFIIS domain (aa 670-790), an SPOC protein-interaction domain (aa 1057-1163), two Pro-rich segments (aa 1257-1286 and 1725-2034) and a C-terminal Arg-rich region (aa 2108-2214). There are least 23 utilized phosphoryation sites. Multiple splice forms exist. Variants are generated by short aa substitutions for aa 531-2240, 566-2240 and 1182-2240, possibly coupled to a 36 aa insertion after Pro387. Over aa 1‑117, human DIDO1 shares 80% aa identity with mouse DIDO1. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human DIDO1 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human DIDO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DIDO1 at approximately 270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
