| 货号 | AF683 |
| 别名 | CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator | 全称 | Receptor Activator of NF-kappa-B |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Agonist Activity(4-8 µg/mL) |
| 目标/特异性 | Detects human RANK in direct ELISAs and Western blots. In these formats, approximately 30% cross‑reactivity with recombinant mouse RANK is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Agonist Activity: 4-8 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8792 (Human); 21934 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-3 inhibits human osteoclastogenesis and bone resorption through downregulation of c-Fms and diverts the cells to dendritic cell lineage. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human RANK Gln29-Gly213 Accession # Q9Y6Q6 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Human RANK/TNFRSF11A Antibody Induces Osteoclast Differentiation in Mouse Splenocytes. Human RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody induces osteoclast differentiation in the presence of 20 ng/mL recombinant mouse M-CSF (Catalog # 416-ML), in a dose-dependent manner, as measured by the tartrate-resistant acid phosphatase (TRAP) solution assay. The ED50 for this effect is typically 4-8 µg/mL. |
