| 货号 | AF6785 |
| 别名 | PAF65B; PAF65BPAF65-beta; PCAF associated factor 65 beta; PCAF-associated factor 65 beta; TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDasubunit 5L; TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associatedfactor, 65 kD; TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associatedfactor, 65kDa | 全称 | TAF5-like RNA Polymerase II p300/CBP-associated Factor-associated factor 65 kDa Subunit 5L |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human TAF5L in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TAF1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 27097 (Human); 102162 (Mouse); 307927 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TAF5L Met1-Tyr189 Accession # O75529 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | TAF5L (TBP-Associated Factors 5-Like; also RNA polymerase II p300-associated factor 65 kDa subunit 5L, PACF65 beta, PAF65 beta and STAF65 beta ) is a 64-66 kDa member of the TAF5 family of proteins. It is a nuclear protein that forms part of the PCAF, STAGA and TFTC histone acetylase complexes. The acetylases within these complexes acetylate hisone H3 within nucleosomes, allowing for the recognition and binding of transcriptional activators. Human TAF5L is 589 amino acids (aa) in length. It contains an N-terminal homodimerization interface (aa 90-198) plus six consecutive WD repeats (aa 266-547). There is one potential splice form that shows a Val substitution for aa 325-589. Over aa 1-189, human TAF5L shares 94% aa identity with mouse TAF5L. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Detection of Human TAF5L by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line, 293T human embryonic kidney cell line, and U2OS human osteosarcoma cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human TAF5L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6785) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TAF5L at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
