| 货号 | AF6780 |
| 别名 | COCA1; DNA mismatch repair protein Msh2; FCC1; hMSH2; HNPCC; HNPCC1; HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1); LCFS2; mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli); MutS protein homolog 2 | 全称 | MutationS Homolog 2 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human MSH2 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4436 (Human); 17685 (Mouse); 81709 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MSH2 Ala2-Asp140 Accession # P43246 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | MSH2 (MutS Homolog 2) is a 100-106 kDa member of the mutS family of DNA mismatch repair molecules. It is a DNA binding protein that is expressed in rapidly proliferating cells, and acts in concert with multiple subunits. MSH2 forms a heterodimer with MSH6, forming MutS alpha. This dimer recognizes and repairs both nucleotide misparings and one or two aberrant nucleotide insertions/deletions. MSH2 also heterodimerizes with MSH3, forming MutS beta. This complex acts on DNA double-stranded breaks, and repairs large nucleotide insertions/deletions (<15 bases). MutS alpha is associated with BLM:p53:RAD51 complexes, while MSH2 beta is associated with SLX4/BTBD12 complexes. Human MSH2 is 934 amino acids (aa) in length. It contains three MutS domains (aa 18-132; 158-284; 297-612) plus an ABC transporter signature motif that may hydrolyze ATP (aa 633-852). There are multiple splice variants. One shows a premature truncation after His429, while others contain a 46 aa substitution for aa 879-934, a 29 aa substitution for aa 462-934, a 28 aa substitution for aa 783-934, a 3 aa substitution for aa 532-934, a 5 aa substitution for aa 482‑934 and a 31 aa substitution for aa 379-934. Over aa 1-140, human MSH2 shares 94% aa identity with mouse MSH2. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human MSH2 by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line and SW480 human colorectal adenocarcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human MSH2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6780) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MSH2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
