| 货号 | AF6748 |
| 别名 | cell growth-regulating nucleolar protein; FLJ20425; Ly1 reactive homolog (mouse); PNAS-5; ZLYAR | 全称 | Ly1 Antibody Reactive Homolog |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human LYAR in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55646 (Human); 170891 (Mouse); 289707 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human LYAR Lys288-Lys379 Accession # Q9NX58 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | LYAR (Ly-1/CD5 antibody reactive clone) is a 45-50 kDa nucleolar protein that was named for the ability of its antibody to cross-react with Ly-1. Its function is unclear; it is known to associate with MYCN and RRP1B, the latter association giving rise to the suggestion that LYAR is involved with RNA metabolism. Human LYAR is 379 amino acids (aa) in length. It contains two C2H2-type Zn finger regions (aa 6-25 and 33-51) followed by one coiled-coil region (aa 175-219) and an NLS (aa 217-222). There are multiple Zn-binding sites and three utilized phosphorylation sites at Ser244, Ser258 and Ser276. Over aa 288-379, human LYAR shares 75% aa identity with mouse LYAR. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human LYAR by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human LYAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6748) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LYAR at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
LYAR in BG01V Human Stem Cells. LYAR was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human LYAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6748) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
