| 货号 | AF6746 |
| 别名 | EC 1.14.11; EC 1.14.11.-; JHDM2A; JHDM2AJMJD1AJHMD2A; JmjC domain-containing histone demethylation protein 2A; JMJD1; JMJD1A; jumonji C domain-containing histone demethylase 2A; jumonji domain containing 1; jumonji domain containing 1A; Jumonji domain-containing protein 1A; KDM3A; KIAA0742; KIAA0742DKFZp686A24246; lysine (K)-specific demethylase 3A; lysine-specific demethylase 3A; testis-specific protein A; TSGA; TSGADKFZp686P07111 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human KDM3A in direct ELISAs and Western blots. In direct ELISAs, approximately 3% cross-reactivity with recombinant human (rh) KDM4A is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55818 (Human); 104263 (Mouse); 312440 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human KDM3A Pro844-Glu968 Accession # Q9Y4C1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | JMJD1A (Jumonji [Japanese for "cruciform/cross"]/ARID domain-containing protein 1A; also KDM3A, JHDM2A, and TSGA) is a 140-150 kDa member of the JHDM2 histone demethylase family of proteins. It is induced by hypoxia, and expressed in spermatids, smooth muscle cells, mammary epithelium, hepatocytes, and renal tubular epithelium. JMJD1A is reported to demethylate di- and mono-methylated Lys9 on histone H3. It has no activity on trimethylated Lys9. JMJD1A is both cytoplasmuc and nuclear, and serves as a coactivator for the AR. Human JMJD1A is 1321 amino acids (aa) in length. It contains one C6-type zinc-finger motif (aa 662-687) and a jumonji C type catalytic domain (aa 1151-1264). There is one phosphorylation site at Ser445. There is one potential splice variants that shows a 13 aa substitution for aa 699-1321. Over aa 810-968, human JMJD1A shares 94% aa identity with mouse JMJD1A. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Lysine (K)-specific Demethylase 3A/KDM3A by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and MOLT‑4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human KDM3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6746) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KDM3A at approximately 150 kDa (as indicated), the band at approximately 70kDa represents the product of degradation or processing. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
