| 货号 | AF6714 |
| 别名 | Cell immortalization-related protein 2; CIR2; CIR2cell growth-inhibiting protein 47; NENF; NEUDESIN; neuron derived neurotrophic factor; Neuron-derived neurotrophic factor; SCIRP10; SCIRP10-related protein; Secreted protein of unknown function; Spinal cord injury related protein 10; SPUF protein; SPUF; SPUFneudesin |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Neudesin in direct ELISAs. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 29937 (Human); 66208 (Mouse); 289380 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Neudesin Gly32-Phe172 Accession # Q9UMX5 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Neudesin (Neuron-derived neurotrophic secreted protein; also CIR2 and SPUF) is a secreted, 20-21 kDa member of the MAPR (membrane-associated progesterone receptor) subfamily, cytochrome b5 family of molecules. It is expressed by CNS neuronal progenitors and neurons, plus preadipocytes in white adipose tissue. Regarding activity, it promotes neuronal differentiation with limited proliferation, and serves as a neuron survival factor. By contrast, it inhibits both astrocyte and adipocyte differentiation. Mature human neudesin is 141 amino acids (aa) in length (aa 32-172). It contains one cytochrome b5-like heme-binding domain (aa 44-129) and an acetylated lysine residue at Lys136. The heme-binding domain does bind heme, and this accounts for 5-6 kDa of its circulating molecular weight. The presence of a heme moiety is necessary for activity. Mature human neudesin shares 97% aa identity with mature mouse neudesin. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Neudesin in Human Brain. Neudesin was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Sheep Anti-Human Neudesin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6714) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
