| 货号 | AF6609 |
| 别名 | E2(25K); E2-25K; EC 6.3.2.19; Hip2; HIP-2; huntingtin interacting protein 2; Huntingtin-interacting protein 2; HYPG; LIG; UbcH1; UBE2K; UBE2K-25K; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-protein ligase | 全称 | Ubiquitin-conjugating Enzyme E2 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat UBE2K/E2‑25K in Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3093 (Human); 53323 (Mouse); 289623 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human UBE2K/E2‑25K Ala2-Asn200 Accession # P61086 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | UBE2K (Ubiquitin-conjugating enzyme E2; also HIP-2, UBE2K-25K and LIG) is a 24-26 kDa cytosolic member of the UBE family of proteins. It is widely expressed (in neurons monocytes and neutrophils), and acts to ubiquitinate proteins targeted for degradation. UBE2K serves as an E2 ubiquitin conjugating enzyme with a preference for ubiquitin chain generation through Lys48. It appears to polyubiquitinate targets once they have been monoubiquitinated, and to act as either a monomer or dimer. Human UBE2K is 200 amino acids (aa) in length and it contains one UBA domain (aa 160-200). There are two potential splice variants that show a deletion of aa 22-72 and 134-176, respectively. Human and mouse UBE2K are identical in aa sequence. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Detection of Human, Mouse, and Rat UBE2K/E2‑25K by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line, U2OS human osteosarcoma cell line, BaF3 mouse pro-B cell line, Neuro‑2A mouse neuroblastoma cell line, and Y3‑Ag rat myeloid cell line. PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat UBE2K/E2‑25K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6609) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, recombinant human UBE2K/E2‑25K (5 ng/lane) was included. A specific band was detected for UBE2K/E2‑25K at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
