| 货号 | AF5414 |
| 别名 | Abelson murine leukemia viral oncogene homolog 1; ABL; ABL1; bcr/abl; BCR-ABL1; c-abl oncogene 1, non-receptor tyrosine kinase; c-abl oncogene 1, receptor tyrosine kinase; c-ABL; EC 2.7.10; EC 2.7.10.2; JTK7; JTK7bcr/abl; p150bcr/c-abl oncogene protein; Proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolog 1; v-abl | 全称 | Abelson Murine Leukemia Viral Oncogene Homolog 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human c-Abl in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 25 (Human); 11350 (Mouse); 311860 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human c-Abl Ala941-Val1140 Accession # NP_009297 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | c-Abl (Abelson murine leukemia viral homolog 1) is a cytosolic member of the ABL subfamily, protein tyrosine kinase family of enzymes. It is ubiquitously expressed, and participates in multiple processes such as cell migration, actin reorganization, DNA damage response and apoptosis. Human c-Abl (I-B) is 1149 amino acids (aa) in length. It is myristoylated on Gly2 and contains one SH3 domain (aa 80‑140), an SH2 domain (aa 146‑236), a protein kinase region (aa 261‑512), three NLSs (aa 620‑634; 726‑739; 778‑791), one DNA-binding region (aa 888‑988), an NES motif (aa 1109‑1119) and an actin F-binding domain (aa 1120‑1149). There is one alternate splice form (I-A) that contains a 26 aa substitution for the N-terminal 45 amino acids. In chronic myelogenous leukemia, c-Abl is fused to the Bcr gene product, resulting in the production of a constitutively active tyrosine kinase. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human c-Abl by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Abl at approximately 135 - 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human c‑Abl by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c‑Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
