| 货号 | AF4924 |
| 别名 | Alkaline sphingomyelin phosphodiesterase; alkaline sphingomyelinase; Alk-SMase; EC 3.1.4.12; ectonucleotide pyrophosphatase/phosphodiesterase 7; ectonucleotide pyrophosphatase/phosphodiesterase family member 7; E-NPP 7; Intestinal alkaline sphingomyelinase; MGC50179; NPP7; NPP-7 | 全称 | Ectonucleotide Pyrophosphatase/Phosphodiesterase 7 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse ENPP-7 is observed, and less than 5% cross-reactivity with recombinant human (rh) ENPP-2 and rhENPP-5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 339221 (Human); 238011 (Mouse); 303729 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human ENPP‑7/Alk‑SMase Ala22-Ser439 Accession # Q6UWV6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (alk-SMase) is expressed in the intestines and in human bile (1). It shares 30%‑36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP-7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies also show a decrease in ENPP-7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP-7 in human colon cancer (4, 5). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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