| 货号 | AF4875 |
| 别名 | calcitonin receptor-like receptor activity modifying protein 3; Calcitonin-receptor-like receptor activity-modifying protein 3; CRLR activity-modifying protein 3; receptor (calcitonin) activity modifying protein 3; receptor (G protein-coupled) activity modifying protein 3; receptor activity modifying protein 3; receptor activity-modifying protein 3; receptor-activity-modifying protein 3 | 全称 | Receptor Activity Modifying Protein 3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human RAMP3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) RAMP1, rhRAMP2, and recombinant mouse RAMP2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10268 (Human); 56089 (Mouse); 56820 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human RAMP3 Arg24-Val118 Accession # O60896 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | RAMP3 (receptor activity modifying protein 3) is a 20-26 kDa member of the RAMP family of proteins. It is expressed on multiple cell types in conjunction with CRLR and NHERF-1, thereby generating a receptor complex for adrenomedullin (AM). Mature human RAMP3 is a 125 amino acid (aa) type I transmembrane glycoprotein that contains a 95 aa extracellular domain (ECD) (aa 24-118) and a 10 aa cytoplasmic region. Although the ECD contains no typical structural motifs, aa 59-65 are critical for AM binding. There is one potential splice variant that shows a 49 aa substitution for the first 19 aa of the signal sequence. The human ECD is 88% aa identical to the mouse ECD. Members of RAMP family of proteins are known to form complexes with apparent molecular weights of 25 to 50 kDa that are resistant to denaturing and reducing agents. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human RAMP3 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RAMP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4875) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP3 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
