| 货号 | AF4476 |
| 别名 | protein S100-A1; S100 alpha; S100 calcium binding protein A1; S100 calcium-binding protein A1S100; S-100 protein alpha chain; S-100 protein subunit alpha; S100 protein, alpha polypeptide; S100A; S100-alpha | 全称 | S100 Calcium Binding Protein A1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human S100A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) S100B, rhS100P, and rhS100A2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6271 (Human); 20193 (Mouse); 295214 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human S100A1 Met1-Ser94 Accession # P23297 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | S100A1 (also S100 alpha) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is expressed by striated muscle, endothelium and chondrocytes. Intracellularly, S100A1 regulates cytosolic Ca levels, and is part of the Hsp70/Hsp90 mutlichaperone complex that protects against protein denaturation. Extracellularly, it exists as both a homodimer and heterodimer with S100B, S100A4 and S100P, may bind to RAGE, and blocks apoptosis. Human S100A1 is 94 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑48 and 50‑85) and a Ca-dependent protein interaction site (aa 89‑91). There may be one alternate start site 53 aa upstream of the standard start site. Full-length human S100A1 shares 93% and 98% aa identity with mouse and canine S100A1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human S100A1 by Western Blot. Western blot shows lysates of human heart and brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4476) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
