| 货号 | AF4396 |
| 别名 | EC 3.1.3.16; MGC21657; Pp2c2; PP2CB; PP2C-beta; PP2C-beta-X; PP2CBmagnesium-dependent, beta isoform; PPM1B; protein phosphatase 1B; Protein phosphatase 2C isoform beta; protein phosphatase 2C-like protein; protein phosphatase, Mg2+/Mn2+ dependent, 1B | 全称 | Protein Phosphatase 2C beta |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse and rat PP2C beta in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PP2C alpha or PP2Cδ. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5495 (Human); 19043 (Mouse); 24667 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PP2C beta Met1-Gly378 Accession # O75688 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protein phosphatase 2C beta (PP2C beta ), also called protein phosphatase, magnesium-dependent type 1B (PPM1B), preferentially dephosphorylates serine and threonine residues on proteins. There are five splice variants with sizes of 53, 43, 42, 36, and 21 kDa. This enzyme is resistant to inhibitors of other protein phosphatases such as okadaic acid and calyculin. Association of PP2C beta with I kappa B kinase inhibits its phosphorylating activity and is regulated by TNFalpha stimulation. PP2C beta is found in many cell types and tissues, especially muscle. The mRNA for PP2C beta accumulates in mature spermatocytes and metaphase-arrested, unfertilized oocytes. Knockout of PP2C beta in mice is lethal prior to embryo implantation. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat PP2C beta /PPM1B by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP2C beta /PPM1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PP2C beta/PPM1B at approximately 53 and 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
