| 货号 | AF4377 |
| 别名 | BHLHB24; bHLHb24DNA-binding protein inhibitor ID-1; Class B basic helix-loop-helix protein 24; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein); dJ857M17.1.2; ID; inhibitor of differentiation 1; Inhibitor of DNA binding 1; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein | 全称 | Inhibitor of DNA Binding 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse ID1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3397 (Human); 15901 (Mouse); 25261 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterization of BMS-911543, a functionally selective small-molecule inhibitor of JAK2. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse ID1 Met1-Glu135 Accession # P20067 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ID-1a is a negative regulator of helix-loop-helix (HLH) DNA binding proteins. ID-1a contains a HLH motif but no DNA binding motif, therefore, upon binding other HLH proteins, ID-1a acts a dominant negative regulator. ID-1a can be found in both cytoplasmic and nuclear cell fractions. Increased ID-1a expression is associated with cellular proliferation and cancer. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human ID1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. Gels were loaded with 20 μg of cytoplasmic (Cyto) and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for ID1 was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
ID1 in BG01V Human Embryonic Stem Cells. ID1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into neural progenitor cells (upper panel), using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
