| 货号 | AF4155 |
| 别名 | integrin beta-6; integrin, beta 6; ITGB6 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Integrin beta 6 in the direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse Integrin beta 6 is observed and less than 5% cross-reactivity with recombinant human (rh) Integrin beta 3 and rhIntegrin beta 5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3694 (Human); 16420 (Mouse); 29302 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Integrin beta 6 Gly22-Asn707 Accession # P18564 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Integrin beta 6 (ITG beta 6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta 6 ECD, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Integrin beta 6 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Sheep Anti-Human Integrin beta 6 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4155, filled histogram) or isotype control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010). | |
Integrin beta 6 in HT‑29 Human Cell Line. Integrin beta 6 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Sheep Anti-Human Integrin beta 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
