Human IRAK4 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human IRAK4 Affinity Purified Polyclonal Ab (100 UG)

货号： AF3919 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF3919
别名	EC 2.7.11; EC 2.7.11.1; interleukin-1 receptor-associated kinase 4; IPD1; IRAK-4; NY-REN-64; REN64; Renal carcinoma antigen NY-REN-64
全称	Interleukin-1 Receptor-Associated Kinase 4
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human IRAK4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IRAK1 and rhIRAK2 is observed.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	51135 (Human); 266632 (Mouse); 300177 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Contribution of globular death domains and unstructured linkers to MyD88.IRAK-4 heterodimer formation: an explanation for the antagonistic activity of MyD88s. Authors: Mendoza-Barbera E, Corral-Rodriguez MA, Soares-Schanoski A, Velarde M, Macieira S, Messerschmidt A, Lopez-Collazo E, Fuentes-Prior P Biochem. Biophys. Res. Commun., 2009;380(1):183-7. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IRAK4 Met1-Ser460 Accession # Q9NWZ3
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that help mediate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. Upon TLR ligand challenge, IRAK4 knockout mice exhibit severely impaired NF-kappa B activation. IRAK4 mutations have been described in patients with recurrent bacterial infections and poor inflammatory responses.
运输条件	Blue Ice
存放说明	-20℃
参考文献	IL-1 Family Intracellular Kinases Intracellular Kinases in the Akt Pathway NOD-like Receptors and the Inflammasome TNF Superfamily Regulators Toll-Like Receptors Translation Regulation Translation Regulation by the Akt Pathway

参考图片

Detection of Human IRAK4 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human IRAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3919) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human IRAK1, IRAK2, and IRAK4 (2 ng/lane) were included. A specific band for IRAK4 was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IRAK4 in THP‑1 Human Cell Line. IRAK4 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Goat Anti-Human IRAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3919) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.