| 货号 | AF3680 |
| 别名 | AAPA3037; EC 1.8.99.-; FGE1; FGEC-alpha-formylglycine-generating enzyme 1; FGly-generating enzyme; MGC131853; MGC150436; sulfatase modifying factor 1; sulfatase-modifying factor 1; UNQ3037 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human SUMF1 in direct ELISAs and Western blots. In these formats, approximately 45% cross-reactivity with recombinant mouse SUMF1 is observed and less than 1% cross-reactivity with recombinant human SUMF2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 285362 (Human); 58911 (Mouse); 362409 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human SUMF1 isoform 1 Ser34-Asp374 with a Ser63Asn substitution Accession # Q8NBK3 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human SUMF-1 (also FGE) is a 42 kDa (341 aa), Ca++-bindingmember of the sulfatase-modifying factor family. It is a soluble ERlumenal glycoprotein that converts inactive sulfatases into an activeform by transforming a catalytic site cysteine into a formylglycineresidue. In the ER, SUMF-1 can exist as either a monomer, or adisulfide-linked homodimer or a heterodimer with SUMF-2. Three spliceisoforms are known. Mature human SUMF-1 shares 90% aa sequence identitywith mouse SUMF-1. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Detection of Human Sulfatase Modifying Factor 1/SUMF1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MCF‑7 human breast cancer cell line, and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Sulfatase Modifying Factor 1/SUMF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3680) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Sulfatase Modifying Factor 1/SUMF1 at approximately 40 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
