| 货号 | AF3526 |
| 别名 | GLI family zinc finger 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; HPE9tax helper protein 1; oncogene GLI2; tax helper protein 2; Tax helper protein; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP; THP1; THP2; zinc finger protein GLI2 | 全称 | GLI-Kruppel family member GLI2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 106cells) |
| 目标/特异性 | Detects human GLI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse GLI-2 and 5% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2736 (Human); 14633 (Mouse); 304729 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. GLI2 Transcription Factor Mediates Cytokine Cross-talk in the Tumor Microenvironment. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GLI-2 Ala2-Glu91 Accession # BAA25668 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GLI-2 is a 166 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least four isoforms that differ in their N- or C-terminal regions have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, human and mouse GLI-2 share 88% amino acid sequence homology. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of GLI‑2-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI‑2/DNA complexes were immunoprecipitated using 5 μg Sheep Anti-Human GLI‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3526) or control antibody (Catalog # 5‑001‑A) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody (Catalog # BAF016). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The bcl-2 promoter was detected by standard PCR. |
