Human BMPR-IA/ALK-3 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human BMPR-IA/ALK-3 Affinity Purified Polyclonal Ab (100 UG)

货号： AF346 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF346
别名	ACVRLK310q23del; ALK-3; ALK3EC 2.7.11.30; BMP type-1A receptor; BMPR1A; BMPR-1A; bone morphogenetic protein receptor type-1A; bone morphogenetic protein receptor, type IA; CD292 antigen; CD292; EC 2.7.11; Serine/threonine-protein kinase receptor R5; SKR5; type II-like kinase 3
全称	Bone Morphogenetic Protein Receptor IA/Activin Receptor-like Kinase 3
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human BMPR-IA in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse BMPR-IA is observed, and less than 5% cross-reactivity with recombinant human (rh) BMPR‑IB and rhBMP RII is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	657 (Human); 12166 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis. Authors: Martinez V, Sacedon R, Hidalgo L, Valencia J, Fernandez-Sevilla L, Hernandez-Lopez C, Vicente A, Varas A PLoS ONE, 2015;10(6):e0131453. Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells. Authors: Lee J, Son MJ, Woolard K, Donin NM, Li A, Cheng CH, Kotliarova S, Kotliarov Y, Walling J, Ahn S, Kim M, Totonchy M, Cusack T, Ene C, Ma H, Su Q, Zenklusen JC, Zhang W, Maric D, Fine HA Cancer Cell, 2008;13(1):69-80. Species: Human Sample Type: Cell Lysates Application: WB Bone morphogenetic protein-mediated modulation of lineage diversification during neural differentiation of embryonic stem cells. Authors: Gossrau G, Thiele J, Konang R, Schmandt T, Brustle O Stem Cells, 2007;25(4):939-49. Species: Mouse Sample Type: Cell Lysates Application: IP Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition. Authors: Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R J. Biol. Chem., 2007;282(32):23337-47. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach. Authors: Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR J. Pathol., 2006;209(2):190-7. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Developmental pattern of expression of BMP receptors and Smads and activation of Smad1 and Smad5 by BMP9 in mouse basal forebrain. Authors: Lopez-Coviella I, Mellott TM, Kovacheva VP, Berse B, Slack BE, Zemelko V, Schnitzler A, Blusztajn JK Brain Res., 2006;1088(1):49-56. Species: Mouse Sample Type: Cell Lysates Application: IP
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human BMPR-IA/AKL-3 Gln24-Arg152 Accession # P36894
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor 1A (BMPR-1A), also known as activin receptor-like kinase (ALK)-3, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IA binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IA is ubiquitously expressed during embryogenesis. In adult tissues, BMPR-IA mRNA is also widely distributed with the highest expression levels found in skeletal muscle. The extracellular domain of BMPR-IA shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IA are highly conserved and share 98% sequence identity
运输条件	Blue Ice
存放说明	-20℃
参考文献	Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.

参考图片

Detection of Human BMPR‑IA/ALK‑3 by Western Blot. Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human BMPR‑IA/ALK‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF346) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BMPR‑IA/ALK‑3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.