| 货号 | AF3255 |
| 别名 | FLJ36569; MGC3245; MSI2; MSI2H; musashi homolog 2 (Drosophila); musashi-2; RNA-binding protein Musashi homolog 2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Musashi-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human Musashi-1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 124540 (Human); 76626 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Musashi-2 isoform 1 Met1-His328 Accession # Q96DH6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Musashi-2 belongs to the evolutionarily conserved Musashi family of RNAbinding proteins which are involved in the translational control oftheir target mRNAs. Musashi-2 has two tandem RNA-recognition domains(RRM-1 and RRM-2) that have the highly conserved RNP(ribonucleoprotein) motifs. By alternative splicing, at least threeMusashi-2 isoforms exist. In mammalian nervous system, Musashi-1 and –2are selectively expressed in neural progenitor cells and play importantroles in maintenance of the stem cell fate. Human Musashi-2 shares 95%amino acid sequence homology with mouse Musashi-2 and 80% amino acidsequence identity with human Musashi-1. Translocations resulting in theformation of Musashi-2/HOXA9 fusion protein is associated withprogression of chronic myelogenous leukemia to the accelerated phaseand blast crisis. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Musashi‑2 in Rat Cortical Stem Cells. Musashi‑2 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human Musashi‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3255) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
